- Hiroshi Kimura
- Yuko Sato
Dynamics of chromatin and epigenetic regulation in vivo
In multicellular organisms, including human, all cells are derived from a single fertilized egg and so essentially harbor the same genetic information. During development and differentiation, specific gene subsets are expressed in specific cell types. As DNA is wrapped around histones to form nucleosomes in eukaryotes, gene expression is regulated at chromatin levels in addition to transcription factors that bind to DNA. In particular, DNA methylation and posttranslational histone modifications play a critical role in "epigenetic" gene regulation. To understand how transcription by RNA polymerase II is regulated by histone modifications and chromatin structure in vivo, we are tracking histone and RNA polymerase II modifications in living cells and organisms by developing new fluorescent probes.
- Stasevich TJ, Hayashi-Takanaka Y, Sato Y, Maehara K, Ohkawa Y, Sakata-Sogawa K, Tokunaga M, Nagase T, Nozaki N, McNally JG, Kimura H. Regulation of RNA polymerase II activation by histone acetylation in single living cells. Nature 2014, 516, 272-275.
- Sato Y, Mukai M, Ueda J, Muraki M, Stasevich TJ, Horikoshi N, Kujirai T, Kita H, Kimura T, Hira S, Okada Y, Hayashi-Takanaka Y, Obuse C, Kurumizaka H, Kawahara A, Yamagata K, Nozaki N, Kimura H. Genetically encoded system to track histone modification in vivo. Sci. Rep. 2013, 3, 2436.
- Hayashi-Takanaka Y, Yamagata K, Wakayama T, Stasevich TJ, Kainuma T, Tsurimoto T, Tachibana M, Shinkai Y, Kurumizaka H, Nozaki N, Kimura H. Tracking epigenetic histone modifications in single cells using Fab-based live endogenous modification labeling. Nucleic Acids Res. 2011, 39, 6475-6488.